Sentence examples for medium in triplicate from inspiring English sources

In order to ensure the stability test of each colony, 100 µL was plated on YPD solid medium in triplicate with different concentration of Kan.

A volume of 100 µL of crude enzyme and uninoculated control was added to separate 300 µL of CMC containing medium in triplicate while blank sample contained 400 µL buffer.

Three disks (5 mm diameter) per leaf were pooled, ground in sterile water, serially diluted and plated on CPG solid medium in triplicate.

Doubling dilutions of serum samples collected from the vaccinated animals were made in 100 µl volume of serum-free growth medium in triplicate wells of 96-well plates and incubated with 102 pfu/100 µl of MVANS3 for 2 hours at 37°C, 5% CO2.

For each concentration 50 μL of compound was added to the medium in triplicate.

Streptomyces strains were cultured in liquid GYM medium in triplicate, as described previously (17).

uPAR-positive and uPAR-negative cells derived from SCLC cell lines (H211, H69AR, H1417) were plated in MethoCult medium in triplicates containing 3×103, 1×103, or 1×102 cells/ml.

To measure IL-12 protein production, 10 THP-1 cells with or without PMA treatment per well are seeded into 1-ml culture medium in triplicates in 24-well tissue-culture plates and incubated at 37°C in humidified 5% CO2 atmosphere in the presence or absence of different stimuli, as indicated above.

Since our aim is to isolate lipid overproducing strains, 25,000 cells from the high-lipid content population of BODIPY 505/515-stained seed cells of CC124 and sta6-1 were sorted directly into TAP medium and were re-suspended in nitrogen-depleted or -replete TAP medium separately (in triplicate).

To validate the prediction of the model, A. oryzae MTG4 was characterized using two optimized media and a non-optimized medium (MEB) in triplicate.

Cells were stimulated with either 10 μg/ml G6PI, 5 μl peptide pool or culture medium alone in triplicate for 72 hours.