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E18 rat hippocampus was removed and dissociated by incubation in 10% [v/v] trypsin-EDTA/growth media for approximately 15 minutes followed by trituration to obtain a single cell suspension.
PBMCs from the three different donors were cultivated independently in these media for approximately three weeks.
Cells were then resuspended in the serum-free delipidated BSA media for approximately 2 h.
These SiNPs were stable in cell culture media for approximately 1 week, and no clumping or aggregation was observed (Nyland et al. 2009; Thomassen et al. 2010).
Approximately 22 h post recovery, the cells were treated with 1000 μM H2O2 for 2 h, then allowed to recover in fresh media for approximately 22 h before analysis.
Pre-cultures of yeast cells containing the tetO-tRNA cassette (tetO-tRNA cells) or the empty cassette (Control cells) were prepared in MMgalactose-URA+geneticin (200 mg/L) media for approximately 16 to 20 hours, at 30°C.
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Briefly, cell pelletes were collected from 80-90% confluent flasks and resuspended in pre-warmed media (50°C) for approximately 6 minutes.
Councillor Mike Layton told the gathering they had originally anticipated about a dozen people, and credited (or blamed?) the media for the approximately 150 that did show up.
Suspension was achieved by first pouring the hot media into the Petri plates, allowing the media to cool for approximately ten minutes in a laminar flow hood, then using tweezers to drop the sterilized wood squares into the media plates.
According to state media this accounts for approximately 2% of China's total farmland.
SAN FRANCISCO, June 4 , 2012/PRNewswire/ — Salesforce.com [NYSE: CRM], the enterprise cloud computing (http://www.salesforce.com/cloudcomputing/) company, today announced that it has entered into a definitive agreement to acquire Buddy Media, the world's leading social media marketing platform, for approximately $689 million payable in cash and salesforce.com equity.
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