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Thereafter the cells were allowed to join the incubator at 37°C and 5% CO2 in their proliferation medium for approximately 24 hours.
Cells were seeded in 96-well plates in 100 ml culture medium for approximately 48 h.
For time-lapse imaging of cldnB:lynGFP myosin6b:mCherry larvae treated with DAPT, embryos at 3 dpf were placed in 50 µM DAPT solution in E3 medium for approximately 6 hr and the solution was replaced during imaging with 100 µM DAPT in E3 medium.
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Slice the tomatoes in half lengthwise, sprinkle with salt and pepper and oil, and roast in a medium oven for approximately 20 minutes.
Cells were therefore grown in rich medium cultures for approximately 8 h, and total cellular RNA was harvested from cells for detection of phaZ genes by RT-qPCR.
A release of I-ANP radioactivity in the culture medium accounted for approximately 80% of the total radioactivity.
To induce differentiation in adipose cells, we grew MABs in adipogenic-inducting medium (Lonza) for approximately 5 days, as described previously.
Boil small to medium potatoes for approximately 7-10 minutes; boil larger potatoes for approximately 12-15 minutes.
Cover the pan with a lid and cook over medium heat for approximately 35 minutes or until the ginger root is tender.
Allow the medium to dry for approximately 30 minutes, though exact dry time depends on how thickly the medium was applied.
Cells cultured overnight in 2 mL of LB medium with 50 mg/L kanamycin were used to inoculate 1 L of LB/kanamycin medium and grown for approximately 3 h at 37 °C with shaking to an OD600 of ∼0.6.
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