Sentence examples for media changed to from inspiring English sources

For experiments, freshly seeded SCs were allowed to attach O.N. in regular media, changed to DMEM containing 10% of newborn bovine serum (NBS) depleted of retinoids (achieved by sequential incubations with AG1-X8 resin and charcoal), in the presence of 2 µM forskolin and 60 µg/ml BPE.

All isolates possessing Lac.2 showed a positive result for lactose fermentation (media changed to yellow), lending further support to the role of Lac.2 in lactose metabolism.

On day 1 of secondary culture (24 hrs after plating) the cell layer was washed in HBSS and the media changed to DMEM supplemented with 10% FBS in place of NuSerum.

Cells were then media changed to either high- or low-glucose media with or without peroxide as appropriate for individual experiments and cultured for a further 8 h before collection of RNA for assessment of changes in gene expression.

The cultures were maintained in Human EpiCult-B (StemCell Technologies) supplemented with 5% FBS (StemCell Technologies) and 50 μg/ml gentamicin for 24 to 48 hours and then the media changed to serum-free conditions and maintained for an additional 10 to 12 days.

Human embryonic kidney (HEK293) and immortalized rat diencephalon astrocyte (DI TNC1) cell lines were transfected in the presence or absence of serum with a pMaxGFP plasmid using either Lipofectamine 3000 or Viromer RED and 6 hr after transfection the media changed to one that either had serum or did not.

After growth in regular media for 24 h and growth arrest with serum-free media for another 24 h, the media was changed to regular media, and BrdU (10 ng/ml; Sigma, St . Louis MO, USA) was added to the media.

After 24 h of incubation, the media was changed to media containing 15 μg/mL blastcidin, and cells were incubated for 5 days.

Cells were seeded in normal media and the day after, media was changed to androgen-free media with charcoal-stripped fetal bovine serum (Invitrogen).

The following day, media was changed to cell maintenance media consisting of plating media without the fetal calf serum and glutamate.

When confluent, the cells were maintained at an air-liquid interface, and the basolateral media were changed to differentiation media.