Sentence examples for medium changed to from inspiring English sources

The cells (105 cells per 24 well) were cultured overnight before addition of tick extract, and the medium changed to RPMI 1640 supplemented with 2.5% FCS and antibiotics.

The cells were incubated for 24 h and the medium changed to 100 µl fresh medium with and without 0.5 µM PPP.

To extend the hematopoietic differentiation, after one week the cytokine cocktail in the differentiation medium changed to a medium containing heparin (5 U/ml, Sigma), TPO (25 ng/ml), human recombinant SCF (25 ng/ml), FLT3L (25ng/ml), IL-3 (10ng/ml), IL-6 (10ng/ml), all from Invitrogen.

Then cells were washed and the medium changed to Claycomb medium 10% FBS.

The next day, cells were washed with PBS PAAandand medium changed to N2B27 without 2i or LIF.

One day before the experiment, cells were washed with PBS (1×), medium changed to HepatoZYME-SFM (Gibco) and cultured for further 24 hrs.

The medium was changed to medium supplemented with 1 mg/ml G418 (Calbiochem) and then re-changed to fresh G418-supplemented mevery every 2 3 days for 2 weeks.

At the end of the exposure the medium was changed to medium containing 10% Alamar Blue and further incubated for 2 h.

The cells were confluent the next day and the medium was changed to medium consisting of 3% FBS, 0.1 mM NEAA, and 2% DMSO.

Twenty four hours post FoxO1, siRNA transfection medium was changed to medium with or without aPKC kinase inhibitor and the cells were incubated for a further 24 h.

Prior to infection, culture medium was changed to medium without serum and antibiotics.