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PHA (5µg/ml) (Sigma, Missouri, USA) as positive control and media as negative control, were added to duplicate wells.
Phytohaemagglutinin (PHA) (5 µg/ml) (Sigma, Missouri, USA) as positive control and media as negative control, were added to duplicate wells.
PHA mitogen at 5 μg/ml was employed as a positive control and culture media as negative control.
Meanwhile, "The Prime Minister's personal refusal of getting vaccinated" and "vaccine's unsafety" appeared in Turkish media as negative news [ 6- 12].
For EPND uptake assay, 1 × 10 LT2-MYC cells per well were seeded on a Nunc Lab-Tek 2-well chamber slide and cultured for 24 h at 37 °C with 5% CO2 and then pretreated with 100 μM dynasore, 50 μM 5- N-ethyl- N-isopropyl) amiloride or 5- N-ethyl-ipin for 30 min. 20 μM of EPND was then added and treated for 30 min with culture media as negative control.
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Masa Urosevic, a high school pupil, shared her experience about social media, as well as the negative experiences of some of her peers, some of whom have been victims of digital violence.
Going negative against the media, as well as her opponent, saved the day for the Clintons.
PHA (10 µg/ml) was included as positive control and media alone as negative control.
Pookweed mitogen (Gibco, Auckland, NZ) diluted at 1/1000 will be used as a positive control and media alone as negative control.
Sixty-nine percent of the parents rated the media as a negative influence on their children's eating habits, followed by the food industry at 61% and the government at 55%.
Homosexuality is widely portrayed in the media as a negative symbol of masculine identity: Homosexuals on television are often promiscuous (Queer as Folk) or serve as the asexual comic character (Will & Grace), but rarely are they presented as masculine men who just happen to be sexually and emotionally interested in other men.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com