Sentence examples for medium as negative from inspiring English sources

For PHA and tetanus antigen, we used culture medium as negative control, and for adenovirus antigen, we used HFF culture supernatant as negative control (Institute of Virology, Ulm University Medical Center, Germany; final concentration 20 μl/ml).

Cells were divided into 4 groups and cultured for 24, 48, and 72 hours with a serum-free modified eagle medium as negative control, modified eagle medium with 25 μg/mL EMD, modified eagle medium with 100 μg/mL EMD, and modified eagle medium plus 10% fetal bovine serum as positive control.

Normal irrelevant rabbit IgG (sc2027, Santa Cruz Biotechnology, CA, USA) was used as internal control, and serum-free medium as negative control.

ESAT-6 and CFP-10 peptide pools were used at 5μg/ml while Phytohaemaglutinin (PHA 5 μg/ml; Sigma, Aldrich, UK) was used as positive control with medium as negative control.

For chemotaxis assay human spermatozoa were diluted at 4×106 cells/ml and then exposed to HAM F-10 culture medium (as negative control), or the attractant gradient (10 pM P).

Spleen cells were also treated with concanavalin A (ConA) (Sigma Aldrich, St .Louis, Mo) at 20 µg/ml (positive control), or with peptide solvent or medium as negative controls.

Three wells were inoculated with 100 μl of fresh inoculum as for MABA and three more wells were incubated with 200 μl of culture medium only, as negative controls.

When sufficient cells were available, 1 × 106 PBMC were incubated overnight with each individual peptide pool or in medium alone as negative control under the same conditions as ELISPOT except for addition of 10 μg/ml brefeldin A (Sigma) after 1 h of incubation.

Eight normal guinea pigs were inoculated with EMJH medium as the negative controls.

The cells were then treated with BCF-CM, NTF-CM or complete medium as a negative control.

Then, the cell cultures were exposed to either 200  μL original extracts of the test specimens or a medium as a negative control.