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One was the mechanical grinding using ball milling with Si3N4 pots and balls.
Mechanical grinding using ball milling with Si3N4 pots and balls was found not to be an efficient way to decrease the grain size because of contamination of Si3N4.
The modern method consisted of roasting cocoa beans with pilot-scale equipment that simulates large-scale chocolate manufacturing using whole bean drum roasters, deshelling the beans followed by mechanical grinding using a ball mill producing a chocolate paste.
Polycrystalline samples of Y x Al y B14 were synthesized using two methods: one is the high-temperature Al flux method [20, 21] with mechanical grinding using ball milling.
Disintegration consisted of mechanical grinding using a scalpel to achieve fragmentation and treatment with enzymes in a digestion medium composed of RPMI, 5% fetal bovine serum (FBS), 1% streptomycin/penicillin,1% L-glutamine (all from PAA Laboratories, Gmb, Germany), 0.1 mM 2-β-mercaptoethanol (Sigma, GmbH, Germany), 1 mg/mL collagenase I (Sigma, GmbH, Germany) and 0.02 mg/mL DNase I.
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The sponges were mechanically ground using an RNAse-free pestle.
Keep grinding, use it as fuel!
Tissue was ground using a mechanical tissue homogenizer and solubilized in lysis buffer provided in the RNeasy Mini Kit (Qiagen USA).
Aortic tissue was ground using a mechanical tissue homogenizer and lysed in a buffer containing 50 mM Tris-Cl, pH 7.4, 1 mM EDTA, 1% Nonidet P-40, 0.1% SDS, 150 mM NaCl, 0.5% sodium deoxycholate, and Halt protease and phosphatase inhibitor cocktail (Pierce Biotechnology, Rockford, IL).
After the solar drying step, fish's samples are then ground using a conventional mechanical mill, which is found at the fermented fish production sites of the area's study.
The sundried seaweeds were ground using a portable grinding machine (Kenstar, Senator, Japan).
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