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Endo-β-1,4-xylanase activity was determined by measuring the reducing sugars liberated from beechwood xylan as previously described (Rakotoarivonina et al. 2012).
The measurement of the enzyme activity from the supernatants of the ΔxlnR, ΔclrB and ΔclrA wheat straw cultures was done using the DNS assay (Miller 1959) measuring the reducing sugar ends released from the incubation of the supernatants with wheat straw.
Cellulase was assayed by measuring the reducing sugar released by reaction on CMC.
Xylanase were assayed by measuring the reducing groups released from beechwood xylan by the dinitrosalicylic acid method (DNS) [ 12].
It is a nonspecific method of measuring the reducing capacity of all the components of the sample other than polyphenols, such as ascorbate [ 18, 36].
The common colorimetric methods currently used for measuring the reducing sugar content are the 3,5-dinitrosalicylic acid (DNS) method and the ferricyanide-based Schales' procedure [ 4, 6, 7].
Similar(54)
As this assay measures the reducing capacity based upon reduction of ferric ion, it is specific to polyphenolic antioxidants.
Ferric reducing antioxidant power (FRAP) generally measures the reducing ability against ferric ion (Fe3+).
The FRAP assay measures the reducing ability of antioxidants against the oxidative effects of ROS.
FDR provides a powerful reference point to measure the dramatically reduced reach of polio today.
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