Exact(4)
To measure the reducing activity of GAE, different concentrations of GAE (25-500 μg/mL) or trolox (50 μg/mL) were used.
The supernatant (hydrolysate liquor) was used in the HPLC analysis to measure the released sugars, and the remaining FP and CL pellets were used in the DNS assay to measure the reducing sugar ends.
The ferric ions (Fe3+) reducing antioxidant power (FRAP) method [ 23] was used to measure the reducing capacity of seed and leaf extracts with a slight modification which involves the presence of extracts to reduce the ferricyanide complex to the ferrous form.
Percent inhibition was calculated from the following equation: (1) % inhibition = (a − b a ) × 100, where a is the absorbance of control and b is the absorbance of test sample The ferric reducing antioxidant power (FRAP) method of Oyaizu [ 21] with little modification [ 22] was adopted to measure the reducing capacity.
Similar(4)
As this assay measures the reducing capacity based upon reduction of ferric ion, it is specific to polyphenolic antioxidants.
Ferric reducing antioxidant power (FRAP) generally measures the reducing ability against ferric ion (Fe3+).
The FRAP assay measures the reducing ability of antioxidants against the oxidative effects of ROS.
Cellulase was assayed by measuring the reducing sugar released by reaction on CMC.
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