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We performed a morphometric analysis of the mitotic spindle by measuring spindle length (pole to pole distance) and spindle width 9 hours following release from a double thymidine block (Fig. 10A 10C), as described previously with GSK3 inhibitors [33].
Instead, interfragmentary motions were indirectly measured by analyzing the motion of the measuring spindle of the dial indicators.
argues that measuring spindle angles using centrosome labeling in 3D is essential to obtain an accurate representation of division orientation distribution.
3) A recent paper by Juergen Knoblich's group (Juschke et al., Proc Natl Acad Sci U S A. 2014 Jan 21 111 3):1014-9) argues that measuring spindle angles using centrosome labeling in 3D is essential to obtain an accurate representation of division orientation distribution.
Figure 1 shows the agreement between the three dial indicators and PONOTS 5 M. For dial indicators 1 and 3 there was no obvious dependence of the amount of measuring spindle motion and the mean differences between the direct (PONTOS 5 M) and indirect measurement (dial indicator).
In order to assess the accuracy of the optical measurement system we attached passive markers of the optical measurement system to the spindle of the dial indicators, analysed the collapse of the measuring spindle and compared the values of the dial indicators with those of the optical measurement system.
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The line measurement tool was used to measure spindle length and width and chromosome distance (chromosome mass length, including the 4th chromosome).
A real time monitoring and diagnosis system to measure spindle center displacement (roundness error) during turning operation is introduced in this paper.
Metaphase was also used to measure spindle size and orientation (Fig. 1).
The freehand ROI tool was used to measure spindle area.
To investigate the relative effects of disruption of astral microtubules and F-actin on spindle orientation, we measured spindle angle (relative to the x/y axis) in control and drug-treated embryos.
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