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Exact(7)
When NIS protein expression was detected by Western blot, NIS protein levels were quantitatively determined from the optical density measurements and normalized to α-tubulin protein levels.
The mean Ct of the gene of interest was calculated from replicate measurements and normalized with the mean Ct of a control gene, GAPDH, for which expression was relatively constant.
The mean fluorescence was calculated from three independent measurements and normalized to respective OD600.
MiRNA-205 expression was determined by 2ˉ△△Ct measurements and normalized to U6 [ 23].
The RNA was quantified (A260 absorbance measurements) and normalized to provide the same amounts of each mRNA sample to the cell free translation mix.
First, the amount of the folded protein was extracted from fluorescence measurements and normalized to that of the folded EmrD (the amount of folded EmrD protein was defined as 1 arbitrary unit).
Similar(53)
They were adhered to the plate by centrifugation at 2000 g for 20 min at 4°C and measurements performed and normalized (after temperature equilibration) as for permeabilized cells but at 32°C.
Values represent three independent measurements and are normalized to the dry body weights; data are presented as means + SEM; n = 3 or 6. * p<0.05, * * p<0.01; two-tailed Student's t test.
All array measurements were processed and normalized using Affymetrix MAS5 algorithm; 13,499 unique gene entries with expression level both above noise and a fold change greater than 1.5 across all tissues were retained.
All array measurements were processed and normalized using Affymetrix MAS4 algorithm, 4,515 unique gene entries with expression fold change greater than 1.5 and standard deviation greater than 40 were retained.
For each pair of primers, triplicate measurements were taken and normalized to input DNA and the amount of DNA recovered from the GAPDH promoter (n > 3).
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