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This work provides guidance on testing and evaluating detection devices, and may contribute to the establishment of suitable standards and normalized procedures.
Absorbance values were compared to standards and normalized by fly weight.
Samples were deproteinized with an equal volume of 10% metaphosphoric acid, centrifuged, and the supernatant was analyzed for GSH and GSSG concentrations using a spectrophotometer at 412 nm over 3 min. Concentrations were calculated by comparison to GSSG standards and normalized to protein content.
Quantification of BH4, BH2, and B was done by comparison with authentic external standards and normalized to sample protein content.
Quantitation of neopterin was carried out by comparison with external standards and normalized for sample protein content.
Analysis was performed using Bio-Rad CFX Manager software and expression values were based on 10-fold serial dilutions of standards and normalized to Actin levels.
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After the incubation time, fluorescence (Exc. 530 nm, Em. 580 nm) was measured and product (resorufin) formed was calculated from calibration standard and normalized with protein concentration.
The quantity of each molecular species was calculated from the ratio of its signal to that of the corresponding internal standard and normalized to the amount of cells.
Each sample was analyzed in triplicates, and the amounts of templates were estimated by linear regression against the known standard and normalized to internal control.
Clones from both standard and normalized libraries are present on the array.
Concentrations were calculated according to the standard and normalized to the seeded number of cells among different samples.
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