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Age, gender, education level and self-reported health were measured as background variables.
Nevertheless, when the fluorescence of DAF-2T is detected, the fluorescence of DAF-2 is measured as background as well.
A 20 μl sample of the incubation medium was measured as reference, and 20 μl from the last wash medium was measured as background control.
For each movie, the mean intensity of an untransfected area was measured as background and was subtracted from the original intensity.
The contours of each pericentriolar area were then manually moved to a nearby extracellular region, and the mean gray level of the enclosed area in the green channel was measured as background (V2).
Similar(55)
To calculate fluorescence values, fluorescence intensity was measured at two points along the edge of a spore and at a point just outside the spore was measured as a background control.
MT1-MMP recruitment to invadopodia over time was measured as the background-subtracted fluorescence intensity of MT1-MMP-pHLuorin at each time point subtracted from the initial fluorescence intensity at the time of invadopodium formation.
Our aim was to evaluate whether SES, here measured as formal educational background, affects the chance of good control of RA during the first year in patients receiving standard care in Sweden.
Artefact was measured as SD of the background attenuation and areas under the curve of SD for each gantry angle acquisition were compared.
Three to six sections were analyzed and the highest expression (measured as hybridization signal minus background) per animal was taken for calculating mean hybridization per nucleus and line [20].
FRET changes were measured as changes in the background- subtracted 480/545-nm fluorescence emission intensities on excitation at 430 nm and expressed as either R/R0, where R is the ratio at time t and R0 is the ratio at time = 0 s, or ΔR/R0, where ΔR = R−R0.
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