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Consumption measured as "Disappearance".
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Supporting this, we observed that co-transfecting the HEK293 cells with USP2b or a catalytically inactive mutant isoform did not affect the rate of degradation of the PER1 protein, measured as the rate of disappearance of the protein following inhibition of protein synthesis with cycloheximide (Fig. 6B).
NADH oxidase activity was measured as the rate of NADH disappearance at 340 nm upon addition 200 μM NADH [ 36].
The intensity of supercoiled DNA band was measured as a function of time, which gave the measure of the disappearance of supercoiled DNA.
The disappearance of GLUT4 myc from the plasma membrane was measured as previously described (Antonescu et al., 2008).
Glutathione reductase (GR) activity (EC 1.6.4.2) was measured as described by Smith et al. [ 82] following spectrophotometrically the disappearance of NADPH at 340 nm (E = 6.2 mM-1 cm-1).
The enzymatic activity was then measured as described by Gibson and Hilf (1983) by a spectrophotometric technique recording the disappearance of cytochrome cred (reduced form of the substrate cytochrome c (Sigma) at 550 nm).
The enhanced air saturations using SCMC for the 1-D air sparging experiment improved the degradative performance of gaseous oxidation agent (ozone) during air sparging, as measured by the disappearance of fluorescence (fluorescein sodium salt).
The rate of ATP hydrolysis was measured by the disappearance of NADH.
IGC, measured by percentage disappearance rate, detects alterations in liver function [ 42].
GRed activity was spectrophotometrically assayed using GSSG as substrate and measuring the disappearance of NADPH at 340 nm [ 46].
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