Sentence examples for mean threshold cycle from inspiring English sources

The mean threshold cycle values for the genes of interest were calculated from three experiments.

For all experiments, the amount of target and control gene was determined from the respective standard curve by conversion of the mean threshold cycle values.

Threshold cycle number of duplicate reactions was determined using the Opticon software and levels of selected gene mRNA expression were normalized to hypoxanthine phsophoribosyltransferase (HPRT) levels using the formula 2 Rt Et), where Rt is the mean threshold cycle for the reference gene HPRT and Et is the mean threshold cycle for the experimental gene.

Relative levels of transcription were determined by using the ΔCt values for each gene obtained by subtracting the mean threshold cycle (Ct) of the GAPDH housekeeping gene from the Ct value of the gene of interest.

A standard curve with serial dilutions of hESC genomic DNA ranging from 0.78 to 25ng/reaction in 2-fold dilution steps was prepared by plotting mean threshold cycle (Ct) values against log-transformed gDNA concentrations.

The mean threshold cycle values from the triplicate of each sample were used for calculations.

Mean threshold cycles for the SpPI and G6pdh reactions were calculated for each sample.

Mean threshold cycles were determined from two technical replicates by the comparative Ct method and expression levels were normalized to GAPDH.

We found good reproducibility as indicated by the intra- and inter-assay CV in the mean threshold cycles (Ct) of a pooled quality control sample of 1.2% and 1.9%, respectively.

The amount of DNA was estimated as follows: 2^(Ct reference Ct sample), where Ct reference and Ct sample are mean threshold cycles of RT-qPCR done in duplicate from GAPDH (reference) and the DNA flanking the I-SceI site (sample).

The percentage of digestion was estimated as follows: 2^(Ct reference Ct sample), where Ct reference and Ct sample are mean threshold cycles of RT-qPCR done in duplicate on DNA samples from GAPDH (reference) and the DNA regions surrounding the I-SceI site (sample).