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The mean green intensity of images from the DGAT1 electroporated leg was significantly higher than observed in the sham-electroporated contralateral control leg (25.77±2.09 AU vs. 8.32±1.68 AU in HFD-DGAT1 vs. HFD-control, p<0.001, and 25.73±3.17 AU vs. 7.37±1.79 AU in CHOW-DGAT1 vs. CHOW-control, p<0.001), indicating significant overexpression of DGAT1.
PDM= (red intensity - mean red intensity)×(green intensity – mean green intensity).
Using image analysis software, the mean green intensity of the nuclear staining was quantified in 10 randomly selected cells.
The mean green intensity of the nuclear staining was quantified with Lucia® image analysis software in 10 randomly selected cells.
The dose effect of each cytokine was evaluated by the measurement of the mean green intensity of the nuclear staining with image analysis software.
ICA analysis generates a correlation/colocalization value for each pixel defined by the Product of the Differences from the Mean (PDM) that is, PDM= (red intensity- mean red intensity) × (green intensity – mean green intensity).
Similar(53)
Besides the naked eyes, two color recognition methods including reading mean green intensities in Photoshop and recording ultraviolet absorbance in microplate reader were also studied.
To differentiate between red and green cells the mean green-intensity of the cell area was thresholded at an intensity value of 50.
The values of the ΔΨm for each time point were expressed as the ratio between the mean red fluorescence intensity and the mean green fluorescence intensity normalized to the correspondent one at T0. Three independent experiments were carried out.
The results showed that the mean green fluorescence intensity and M RNA transcripts were significantly reduced, and that the expression of M glycoprotein was strongly inhibited in those cells co-transfected with M-specific siRNAs.
The values of mitochondrial mass for each time point were expressed as the ratio between the mean green fluorescence intensity and the one correspondent to time zero (T0).
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