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PDM= (red intensity - mean red intensity)×(green intensity – mean green intensity).
Selected sections were analyzed using the histogram function through the red channel, which gave the mean red intensity of the selected section.
ICA analysis generates a correlation/colocalization value for each pixel defined by the Product of the Differences from the Mean (PDM) that is, PDM= (red intensity- mean red intensity) × (green intensity – mean green intensity).
Similar(57)
The values of the ΔΨm for each time point were expressed as the ratio between the mean red fluorescence intensity and the mean green fluorescence intensity normalized to the correspondent one at T0. Three independent experiments were carried out.
Detected spots with a mean green intensity <20 were omitted and spots with a mean red fluorescent intensity <50 were classified as released cores (ntotal ∼750 cores in 10 cells/condition and experiment).
AGE deposition was quantified in the arterial media by measuring mean red fluorescence intensity (range 0 255, arbitrary units) in the analyzed area and expressed as the mean fluorescence intensity for each vessel.
Phagocytosis was calculated from the drop in mean red fluorescence intensity of green-positive cells after incubation for additional1h at 37°C as described [25].
HeLa cells treated with Surv-T34A, but not Surv-WT, showed a time-dependent reduction in the mean red fluorescence intensity.
A reduction in Δ ψm results in diffusion of the dye from the mitochondria and a subsequent reduction in the mean red fluorescent intensity.
Green color represents probes with intensity less than mean; red color represents probes with intensity more than mean.
She does a mean red.
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