Sentence examples for mean florescence intensity from inspiring English sources

Results were expressed as positive cells ratio and mean florescence intensity.

Teff cells expressing high levels of αL or α4 integrin on their surface (αLhi or α4hi cells) were defined as the cell population staining with a mean florescence intensity above 102 (gated on activated lymphocytes).

Mean florescence intensity (MFI) was calculated using CellQuest Pro software (BD Biosciences) with a total of 15000 cells counted.

Percent change in fluorescence is calculated as ((Fx F0)/F0 × 100%) where Fx is the mean florescence intensity at time point x and F0 is the starting fluorescence at time=0.

Under our experimental conditions, it is possible to consider TAMRA-positive phagocytes as phagocytes internalizing apoptotic cells (Supplementary Figure 1), and the TAMRA mean florescence intensity (MFI) of engulfing phagocytes represents the relative number of apoptotic cells engulfed per phagocyte.

Whilst exposure to FITC-labeled fMLP resulted in specific binding of FITC-fMLP on neutrophil membranes by flow cytometry and 6(b), pre-exposure of cells (1 × 10/mL) to unlabeled fMLP prevented FITC-fMLP binding thereby reducing the mean florescence intensity reading by approximately 75% (40.05 ± 4.7 to 11.04 ± 1.45 MFI, Figures 6(a) and 6(b)).

Shown are the mean florescence intensities of the GFP+ cells (white line) and GFP- cells (black line) from the same sample.

For analysis, cells were washed with PBS, fixed using 4% paraformaldehyde, permeabilized using 0.1% Triton X-100 in PBS, blocked, and then incubated with Anti-phospho-S6K antibodies in LI-COR blocking reagent overnight and average florescence intensity (in arbitrary units) of each well was quantitated using ODYSSEY infrared imager (LI-COR Biotechnologies).

All of these negative samples had lower median florescence intensity (MFI) values (294 825) in the RVP Fast assay, suggesting that the GeXP assay has a slightly decrease sensitivity for the detection of HMPV, Adv, RSVB and HBoV.

The florescence intensity values were normalized to protein levels in the supernatants.

Clones of DLD-1/SV40-RLuc were produced using two different techniques (96 well plates and Petri dish) and their florescence intensity was recorded using IVIS machine (Calliper Life Sciences, Hopkinton, USA).