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Mean fluorescent intensity (MFI) was calculated as a ratio of mean fluorescence sample/isotype fluorescence.
Mean fluorescent intensity (MFI) was determined by the geometric mean of fluorescence of the cells and unstained cells served as a negative control.
Data were analyzed using fluorescence-activated cell sorting (FACS) analysis and shown as mean fluorescent intensity (MFI).
Mean fluorescent intensity was determined with the ImagePro 6.2 software after normalizing to background fluorescence.
The mean fluorescent intensity (MFI) was measured with flow cytometry.
(F) Bar graph quantification of the mean fluorescent intensity analyzed using the FlowJo software v7.6.1.
(D) Bar graph quantification of mean fluorescent intensity analyzed using the FlowJo software v7.6.1.
Fig. 4 Correlation between the mean fluorescent intensity (MFI) of PMAP-1 and polymorphonuclear neutrophil (PMN) activation (CD11b expression).
Fig. 3 Mean fluorescent intensity (MFI) of PMAP-1, MitoAP-1, and CD11b expression in PMNs in sepsis patients and healthy control (HC) subjects.
Monocyte human leucocyte antigen-DR (mHLA-DR) membrane density (geometric mean fluorescent intensity) was characterised using flow cytometry following a 20-hour incubation.
Mean fluorescent intensity (MFI) and percent of positive cells were measured.
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