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Here we analyze the sequences, structures, and evolution of LPMOs to understand the factors that may influence substrate specificity both within and between these enzyme families.

Interestingly, the ECE-like group of enzymes (ECEL-1, DINE, etc), for which there are no known physiological substrates, are unique in that all have a LNAYY motif, which may influence substrate specificity.

The colonization of trees by non-vascular epiphytes (bryophytes and lichens) may influence substrate suitability for vascular epiphytes and, thus, cause host biases (Pollard 1973; Sanford 1974; Zotz 2002).

The availability of a crystal structure for P. marinus cADO (strain MIT9313; Joint Center for Structural Genomics PDB IDD: 20C5) enabled us to investigate the active site architecture, and identify residues that may influence substrate binding.[ 10] The structure reveals a mainly alpha helical architecture, with a ferritin-like four-helix bundle.

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Thus, the T311M mutation may indirectly influence substrate interactions with these loops.

The A188T mutation may thus influence substrate binding through the L2 loop.

However, it lies in a predominantly positively charged region of the protein that may influence its substrate specificity.

Thus, pharmacokinetic issues may play a role when adding substrates but also pharmacodynamic factors (e.g. effects of the substrate) may influence ROS assay results.

The physicochemical property of substrate may influence the attached microbial community structure (Dang and Lovell 2000).

A potential problem for the atmospheric pressure plasma treatment is that the moisture absorbed by the substrate may influence plasma surface modification processes.

We also discuss the possibility that factor(s) other than ADH/ALDH genotype may influence activity at low substrate concentrations.

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