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Exact(4)
Disruption of chorismate mutase/prephenate dehydratase (pheA) to preserve endogenous chorismate then allowed maximum titers of 277 ± 2, 454 ± 11, and 451 ± 44 mg/L, respectively, at glucose yields of 5.8, 9.7, and 14.3% of their respective theoretical maxima.
All the recombinants grew well in cell culture, although the maximum titers of rNiV(V−) and rNiV(C−) were lower than the other recombinants.
To achieve a one-step infection of all cells, MOI was chosen between 3 (NIBSC) and 20 (RKI) depending on maximum titers of available virus seeds (2.1 × 107 virions/mL, NIBSC; 3.2 × 109 virions/mL, RKI).
In view of the 1.8 and 2.2 fold respective increases in qp and maximum titers of the pAID cell pool used for normalization, this verified our previous postulate that the rhA1AT transgene had minimal gene amplification and as such lowered relative transcript copy numbers, in the 4 pAIDp and pAI772Dp cell pools during the increase in MTX concentrations from 50 nM to 300 nM MTX.
Similar(56)
In addition, 28 subjects with pre-existing rHuPH20-reactive antibodies prior to exposure experienced treatment boosting, as defined by an increase in titer of two titering steps or more following treatment, and one of these experienced a maximum titer of 81,920, the highest titer observed following rHuPH20 exposure in this data set.
In comparison, the maximum titer of wild type C. thermocellum was 14.1 mM ethanol on 10 g/L Avicel.
The maximum titer of AMVL was approximately 60 mg/L in the MEB medium, whereas AMVL was approximately 200 and 250 mg/L in the optimized defined and complex media, respectively.
Using a stepwise optimization, an overall whole-cell biocatalytic process was developed and a maximum titer of 260.0 mM Neu5Ac (80.4 g/L) with a conversion yield of 43.3% from GlcNAc was achieved.
Cultivation of the best-performing strain in YG medium and a controlled bioreactor set-up resulted in a maximum titer of > 4 g L−1 1,2-PDO which, to the best of our knowledge, has been the highest titer of 1,2-PDO obtained in yeast so far.
Further MTX amplification to 300 nM MTX gave a maximum titer of 1.15 g/l.
As a result of more amplification, the pAIDpp Pool 1 also attained a g/l maximum titer of 1.15 g/l, comparable to that of the pA772Dp Pool 1 at 1.11 g/l.
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