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This negative vaccinate has the highest titers of IgA in saliva and systemic IgG against PrP.
Highest titers of 2.59 g/L for 2-mthylbutyric acid and 2.58 g/L for valeric acid were achieved in shake flask experiments through optimal combinations of these enzymes.
In these strains, MccJ25 production does not commence until the cells reach stationary phase, and, according to previous literature, the highest titers of MccJ25 are obtained from cells grown in nutrient-poor medium.
First, comparisons of various exogenous α1,2-fucosyltransferase and α1,3-fucosyltransferase candidates, as well as a series of E. coli host strains, demonstrated that futC and futA from Helicobacter pylori using BL21 DE3) as the host strain yielded the highest titers of 2′-FL and 3-FL.
Of the panel of tested peptides, a chimeric peptide consisting of two copies of a T-helper epitope (residues 288 302 of MVF) and one copy of the mimotope M2 (TTM2) and a multiple antigen peptide with eight copies of M2 (MAP-M2) induced the highest titers of anti-M2 and anti-MV antibodies.
A total fatty acid contents of up to 16% (w/w) was obtained on complex media, corresponding to approximately 9% (w/w) triacylglycerols and representing the highest titers of fatty acids and triacylglycerols obtained in E. coli under comparable cultivation conditions, so far.
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To the best of our knowledge, this system yielded the highest titer of 2-PE ever obtained in E. coli.
Improving the NADPH availability via the ΔpgiΔppc mutation, BLΔpgiΔppc-p148 produced the highest titer of catechin at 760.9±84.3 mg/L.
The highest titer of 1 1120 was found in one sample.
Finally, the tryptone culture achieved the highest titer of FFAs (716.1 mg/L).
Post-immune sera contained high titers of anti-BET IgG.
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