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The maximum substrate degradation efficiency of 96.9% was estimated at the temperature of 39.3 °C, the initial pH of 7.0 and the glucose concentration of 26.8 g/L.
Higher cumulative H2 production and maximum substrate degradation were also noticed with BEH-pretreated substrate (CHP, 0.91 l; COD, 52.42%) than control (CHP, 0.68 l; COD, 43.68%).
Acetate was obtained as a major metabolic intermediate from the degradation of glucose and a shift in the biochemical pathway towards formation of butyrate occurred after maximum substrate degradation.
The maximum substrate degradation efficiency, the maximum hydrogen yield and the maximum average hydrogen production rate was predicted at the temperature of 37.8 °C and the initial pH of 7.1, 37.4 °C and 6.9, and 38.2 °C and 7.2, respectively.
The maximum substrate degradation efficiency of 95.3%, hydrogen yield of 305.3 mL/g glucose and average hydrogen production rate of 23.9 mL/h were all obtained at the optimal temperature of 39.0 °C, initial pH of 7.0 and glucose concentration of 24.6 g/L identified by the method of desirability function based on a neural network.
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In particular, the strategy of Kinetic Control coupled with Minimal Medium (MM-KC) led to a maximum ethanol yield of 4.6 g/L, together with a 1,3 PD yield of around 3 g/g, with complete substrate degradation within 21 h.
We further investigated the substrate degradation with SEM.
The mechanisms of substrate degradation and recognition remain unknown, and this protein may be useful in future investigations of the substrate degradation and recognition mechanisms of C. cellulovorans.
Scanning electron microscopy [SEM] was used to investigate the substrate degradation and morphology of cells grown on the different substrates.
Vacuolar staining is absent because of rapid substrate degradation there.
A high degree of substrate-substrate interaction could reduce enzyme accessibility to individual alginate chains and slow substrate degradation relative to non-acetylated BSWA.
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