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The maximum ion time was 200 ms for MS and MSn.

The LTQ was used to acquire MS/MS spectra (3 m/z isolation width, 35% collision energy, 5,000 AGC target, 200 ms maximum ion time).

The Orbitrap was used to collect MS scans (300 to 1600 m/z, 1,000,000 AGC target, 1,000 ms maximum ion time, resolution 30,000).

The AGC (automatic gain control) maximum ion time was set to 500 msec (but typically utilized <10 msec) and five " μscans" were acquired for each saved spectrum; thus, the cycle time for each transformed and saved spectrum was about 10 sec.

Similar(56)

Tandem MS were collected in the high-pressure linear ion trap (MS/MS) with the following settings: ion threshold, 5000; minimum intensity, 3000; maximum ion accumulation time, 25 ms; and activation time, 10 ms.

MS analysis was performed in data-dependent MS/MS scan mode, spray voltage was 2.0 kV, MS full scan range was 300 1600 m/z, AGC was set as 3e 6, resolution at 400 m/z was 70,000, the maximum ion injection time was 60 ms.

Maximum ion accumulation time was 500 ms for full MS scans and 100 ms for MS2 scans.

For the analysis of unlabeled peptides, the instrument method consisted of a full MS scan (scan range 375 1550 m/z, with 30K FWHM resolution @ m/z = 400, target value 2×106, maximum ion injection time of 500 ms) followed by data-dependent CID scans of the six most intense precursor ions.

Maximum ion injection time was 100 ms, and normalized collision energy was 35%.

For MS and MS/MS spectra acquisition, the maximum ion inject time was set as 500 and 250 ms, respectively.

Automatic gain control (AGC) was set to 5 × 10 and maximum ion injection time (IT) was set to 200 ms.

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