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Maximum ion accumulation time was 500 ms for full MS scans and 100 ms for MS2 scans.
Target values were set at 5×105 and 104 for the survey and Tandem MS scans, respectively, and the maximum ion accumulation times were set at 200 ms in both cases.
The maximum ion accumulation time was set to 100 ms for MS and 300 ms for MS/MS scans.
Maximum ion accumulation times were 1000 ms for each full MS scan, 150 ms for MS2 scans, and 250 ms for MS3 scans.
The ion selection threshold for the Orbitrap (MS) was set at 1.0 × 10 with a maximum ion accumulation time of 500 ms.
Tandem MS were collected in the high-pressure linear ion trap (MS/MS) with the following settings: ion threshold, 5000; minimum intensity, 3000; maximum ion accumulation time, 25 ms; and activation time, 10 ms.
Similar(54)
The ion selection threshold was 500 counts and the maximum allowed ion accumulation times were 500 ms for full scans and 250 ms for HCD.
Survey full scan MS spectra (from m/z 300 to 2000) were acquired in the Orbitrap with resolution R = 60000 at m/z 400 (after accumulation to a target value of 1e6 in the linear ion trap with maximum allowed ion accumulation time of 500ms).
A maximum injection time of 60 ms was used for ion accumulation.
Ion accumulation was set to 180,000 with accumulation maximum of 200 ms.
: which is appropriate for simple ion accumulation mechanisms.
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