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This method allows the user to set minimum median and maximum fragment length used in the original ChIP-seq experiment.

Pairwise P values are assigned based on the comparison of each fragment with the maximum fragment length that is expected from the sequence pair by chance.

However, the maximum fragment length and sequence divergence between homeoloci where HRM remains useful for SNP or mutation detection is unknown and further experiments are required.

Alignment of amino acid kinesin-motor-domain sequences was performed using DIALIGN-T 0.1.2 [ 53] with the default settings (length of a low-scoring region = 4; maximum fragment length that is allowed to contain regions of low quality = 40).

Paired reads were aligned to the bovine genome (UMD 3.1.69) with Bowtie2 (version 2.0.5), using a maximum fragment length (−X) of 500 and minimum fragment length (−I) of 0 for valid paired-end alignments.

For each RNA-seq replicate (4 per strain, 8 in total), reads were mapped against this concatenated sequence using Bowtie version 2.1.0 [ 81] with the preset option "--very-sensitive-local" and with the maximum fragment length for valid paired-end alignments set to 1000 (−X 1000).

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Similar(52)

The distance used is the approximate maximum expected fragment length (p-value ≈ 0.005) in the clusters of supporting RP fragments.

Therefore, given the mapping of the ith RF read pair (i.e., e i and s i ) and the minimum and maximum values of the fragment length, lmin and lmax, we can define the range of possible start and end breakpoints of the tandem duplication that induce the ith discordant mapping using the inequalities (i), (ii), (iii) and (iv).

ISSR: Inter simple sequence repeat; PCR-RFLP: Polymerase chain reaction-restriction fragment length polymorphism; MP: Maximum parsimony; ML: Maximum likelihood; NJ: Neighbor-joining; UPGMA: Unweighted Pair Group Method with Arithmetic mean; NCA: Nested clade analysis; IBD: Isolation by distance; LGM: Last glacial maximum.

The parameter value u = 2 was chosen to specify the maximum variation among fragment lengths originating from TDFs determined (by eye) to be identical.

The frequency distribution of the maximum fragment diameter indicates an average conch length of the Isorthoceras specimens of 80 90 mm.

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