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The increased fragment length is necessary to accommodate paired end sequence analysis.
Amplification across the exon 6 7 boundary confirmed an increased fragment length in the cDNA of these white flowered lines, consistent with the proposed mis-splicing (Fig. 2D).
Insertion mutations were scored when peaks of at least 20% the height of the wild-type peak closest to the presumed mutant peak was observed in a position that indicated increased fragment length and that did not correspond to the peaks observed in undiluted DNAs obtained from blood lymphocytes of the same subject (for example Figure 1C, third panel).
Similar(57)
These fragments, called restriction fragment length polymorphisms (RFLPs), often serve as effective "genetic markers".
Amplified fragment length polymorphism.
restriction fragment length polymorphisms.
Fragment length polymorphism.
PCR-restriction fragment length polymorphism.
terminal restriction fragment length polymorphism.
terminal fluorescence labeled-restriction fragment length polymorphism.
Polymerase chain reaction-restriction fragment length polymorphism.
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