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This metal cannot undergo metabolic degradation, is poorly excreted, and is retained in the liver with a very long biological half life (20 30 years) [ 2], thus providing us with the fact that the maximum concentration used (10  μM) may be compatible with bioaccumulation in the liver.

The results show that after a short obligatory preliminary experiment, simulations provide users with the best experimental conditions to be used, in particular, the maximum concentration used to reach saturation, the dilution factor for the serial dilutions of the sample injected and the duration of the dissociation and association phases.

A subset of wells was used to determine cellular toxicity of the inhibitors SP600125 and U0126 at the maximum concentration used in the dose response study, 35 µM and 20 µM respectively.

The fold-shift for VU0152100 at 1 μM (the maximum concentration used for VU0467154) was approximately 23-fold.

Glucose, at the maximum concentration used in the assay (2 mM), did not significantly alter the sensor response (data not shown).

MBCs of most of the extracts the plant found to be higher than 100 mg/ml which was the maximum concentration used for the experiment (Table  3).

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The maximum concentrations used for wet spiking were limited by the maximum water holding capacity (WHCmax) of the soil and by the maximum concentrations suitable for the stock dispersions.

The maximum concentrations used in this study were 10 µM and found to be the ideal dose for in vivo inhibition of HDAC activity in the colonic mucosa [38].

We chose 25NAA/25Kin and 300NAA/300Kin, the minimum and maximum concentrations used in the grids.

At the maximum concentrations used in this study, the predicted mole-fractions ranged from ∼0.01 to ∼0.05, close to the 0.1 mol-fraction used in MD simulations.

We treated OVCAR5 cells with vehicle (mock), 675  μ I3C, 37.5 n bortezomib or combination for 24 h, identical to the maximum concentrations used for our apoptosis and cell cycle studies.

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