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The results indicate that 25000-fold dilution of the antiserum (maximum dilution used in this experiment) was still sufficient to detect the 6 ng band.
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The dilution used was 1: 500.
Dilution used for immunocytochemistry: 1/5.
Dilution used for actin labeling: 1/100.
Titers were calculated as the dilution corresponding to 50% of the maximum absorbance from a plot of the absorbance versus log(dilution) using GraphPad Prism 5. Dissociation constants and antibody concentrations were determined using competitive RIA.
A maximum dilution of ¼ was used so that the maximum concentration measured with the haptoglobin SRM assay was 4600 mg/L.
It is performed by using maximum dilution of antibody which can confirm 90% reduction in viral infectivity after virus neutralization [ 10].
The dilutions used in a serial dilution test determine which concentrations it can estimate well.
What I can say now is that the high dilutions (used in homeopathy) are right.
The 10 dilution of the NP enrichments and the 10 dilution of NP + P enrichments were the most diluted 4- n-NP degrading enrichments, and were used for a second 10-fold dilution series with a maximum dilution of 10 (fifth generation).
The difference was calculated as, log2(maximum dilution of the repeated test) – log2(maximum diluation of the baseline test).
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CEO of Professional Science Editing for Scientists @ prosciediting.com