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With the remaining trees a maximum clade probability tree was calculated using TreeAnnotator v1.7.5 [ 86].
Alternative maximum clade probability tree, inferred from the analysis of Cyt b data.
The maximum clade probability tree (Fig. 2) inferred in BEAST supports the Garli and MrBayes phylogenies.
For the chronogram obtained under bayesian analysis, we calculated the γ statistic for the maximum clade probability tree with mean node heights.
See Fig. 2 for the corresponding maximum clade probability trees As Clade A was only comprised of two individuals, statistical comparisons were only made between animals belonging to Clades B and C. Shapiro Wilk's test (P > 0.05) and a visual inspection of their histograms showed that the variable characters in the dataset were normally distributed.
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The tree with the maximum product of the posterior clade probabilities (maximum clade credibility tree) was chosen from the posterior distribution of 10 001 sampled trees after burning in the first 1000 sampled trees with the program TreeAnnotator version 1.6.2.
The trees were summarized in a target tree by the Tree Annotator program included in the Beast package by choosing the tree with the maximum product of posterior probabilities (maximum clade credibility) after a 10% burn-in.
The maximum clade credibility tree with posterior probability of each node was computed with the program TreeAnnotator (BEAST v 1.6.0).
The sampled posterior trees were summarized using TreeAnnotator ver. 1.4.8 [ 24, 37] to generate a maximum clade credibility tree (maximum posterior probabilities) and calculate the mean ages, 95% highest posterior density (HPD) intervals, posterior probabilities (PP) and substitution rates for each node.
The sampled posterior trees were summarized using TreeAnnotator v.2 to generate a maximum clade credibility tree (maximum posterior probabilities) and calculate the median ages and 95%% highest posterior density (HPD) intervals for each node.
The first 25% of the generations were discarded as the burn-in, the maximum clade credibility tree and corresponding posterior probabilities were calculated using TreeAnnotator [81].
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