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The phosphorylation probability is determined based on a pre-defined maximum phosphorylation probability, kphr.
The allosteric parameter can take any value between 0 and 1, and is multiplied by the maximum phosphorylation probability.
To calculate the reaction probability, the allosteric parameter was multiplied by the maximum phosphorylation probability, kphr, which is 0.1 for stress-free and 1 for stressful conditions.
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Maximum phosphorylation was found after 15 and 30 min for Ser536 and Ser276, respectively (Figure 2A).
For tyrosine 1068, the onset of maximum phosphorylation was similar.
Only if the phosphorylation probability was smaller than the random number was phosphorylation deemed to have occurred.
Therefore, we chose 10 minutes (maximum phosphorylation of Smad2/3) as the stimulation time for TGFβ3.
To characterize all genes assigned into one of the nine categories, we apply three assignment rules: maximum probability, maximum probability over 0.6 and maximum probability over 0.7.
(OHT v.s. WT) Three values in each cell present the number of genes belonging to each category using three different assignment rules: maximum probability, maximum probability over 0.6, and maximum probability over 0.7.
More significantly, the maximum interchange probability is high, which is 0.46 for H5N1 NS1 protein(Figure 3D).
In addition, for fixed posterior probability, the maximum prior probability compatible with a given P value can be read off.
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