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Their method consists of dominant edge lines for global transformation estimation and Harris corner points (Harris and Stephens 1988) with maximal response for subsequent local transformation refinement.
For cells that showed supersaturation (n = 13/81), we removed the data points that were below the maximal response for contrasts larger than the one evoking the maximal response and the fit was made on this reduced data set.
Interestingly, similar features to the present constitutively active receptors have been described: This includes the observation that the overexpressed Ste2-P258L, S259L -mutant receptor shows PFUS1-reporter levels comparable to the maximal response for α-factor-activated native Ste2p [24], despite only weak induction of the expected "shmoo -morphology.
Currents from individual patches are normalized to the maximal response for each patch.
The maximal response of tissue (Emax) was calculated as a percent of the maximal response for PHE.
The dose of capsaicin to elicit a maximal response for both OAB and control cells was 10 μM.
Similar(46)
Similarly, run training alone (TR group) provoked an increase of the maximal responses for ACh (10%).
The position of the point in the center lies within the domain of maximal responses for yield and initial conversion rate, explaining why no other points with significant improvements can be found.
Stimulation by concentrations of G-1 and estrogen as low as 1 and 10 nM, respectively, yielded significant increases in FOXO3-GFP translocation with maximal responses for G-1 and estrogen obtained at ~100 nM and 50 nM, respectively, which are the concentrations utilized throughout the remainder of this study.
The coupled Fb/H-35 cell assay also allowed quantification of IL-1 in which the dose-dependent induction of IL-6 expression by 10-fold serially diluted Ep CM was compared with that of 10-fold serially diluted IL-1 α or IL-1 β standards (half-maximal response for both IL-1 forms at ∼0.1 ng ml−1).
To investigate the effects of perinatal BPA exposure on the response to E2 at puberty, we used the dose response curves of the morphometric points described above to identify two doses higher than the LOEL but lower than the half-maximal response for each strain.
Related(20)
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