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These coating concentrations promote maximal adhesion for each cell line as shown in Figure 1.
Maximal adhesion for HT29 occurred already after 12 h preincubation of MEC with X XO and was 167% vs control (P<0.01).
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Maximal adhesion of FcRγ−/− platelets was to GFOGER, which was 30% of WT adhesion to CRP.
The lowest cell adhesion was detected in α11-shRNA hMSC as these cells reached a maximal adhesion of just 30.1±3%.
Maximal adhesion was observed to CRP.
Interestingly, adhesion assays show that tumour cells adhere preferentially to LEC with maximal adhesion exhibited at 30 to 40 minutes.
No significant difference in the adhesion rates of cells expressing either EGFP-paxillin or EGFP-S188/190A was observed, both cell types achieving half-maximal adhesion at 0.5 μg/ml, with maximal adhesion at 2.5 μg/ml.
Figure 6 shows that control adhesion of TK-1 cells was about 57.68 ± 3.77 of the maximal adhesion induced by TNF-α.
Figure 4 showed that control adhesion of TK-1 cells was 12.3 ± 0.98%; TNF-α induced maximal adhesion (29.8 ± 1.30%, p < 0.001 vs. untreated control) at 24 h.
We demonstrated that maximal adhesion was obtained at a SDF-1α concentration of 200 ng/ml (Fig. 3a).
TNF-α induced maximal adhesion (100 ± 2.1%) which was significantly elevated compared to controls (p < 0.01TNF-α vs. control).
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