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Maximal projections of Z-stacked images were obtained and analyzed with ImageJ software.
Figure 6 shows XY and ZY maximal projections of approximately half the field of view of a typical image stack.
Images shown from confocal are 2D maximal projections of z-stacks.
Analyses of inclusions in cultured neurons were performed on maximal projections of confocal stacks.
To display the entire cell volume, images are presented as maximal projections of z-stacks using Axiovision 4.8.2 (Carl Zeiss).
Maximal projections of z-series stacks were made with Volocity (http://www.improvision.com) or ImageJ (http://rsb.info.nih.gov/ij/) software.
Similar(49)
Z-stack images were output as a maximal projection of multiple z-planes.
For each oocyte, a maximal projection of 10 planes was performed.
Images are, unless specified, maximal projection of Z-stacks performed using a 0.2 or 0.3 µm step.
Mean signal intensity of the maximal projection of the apical membrane of the intestine (from the pharyngeal-intestinal valve to the rectum) was measured using the freeware ImageJ.
(A ) Flat maximal projection of the membrane protein PIN1 GFP in an Arabidopsis inflorescence meristem using ImageJ.
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