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Maximum projections of stacks are shown (Figure 4B).
Maximum projections of the fluorescence images were generated and coloured using ImageJ software.
Unless stated otherwise, representative images shown are maximum projections of Z-stacks taken with 0.20µm increments capturing total cellular volume.
In some cases, maximum projections of CSLM image stacks revealed the connections with the subtending mesophyll cells (example illustrated for At1g56145 in Fig. 5A).
Z-series were performed with a Z-step of 1 µm and maximum projections of Z planes including the spindle region are displayed.
Maximum projections of en face views of endplates were taken, and five neighboring lateral axes were used to measure fluorescence intensities at every point and to obtain average values along these axes.
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Both the maximum projection of rainfall intensity and the maximum change in future projections are affected by using different reference periods for different frequencies and durations.
Leica software (Application Suite 1.8.0) was used to create a maximum projection of the z-stack.
Maximum projection of z-stacked spinning-disk confocal images is shown.
(A – H ) Maximum projection of tonoplast localised SNAREs and marker protein NET4A under high auxin conditions.
On the right is a maximum projection of frames from 5 s of real time.
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