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Fo: The maximal fluorescence of light adapted samples.
We have previously determined that the maximal fluorescence of FLICA reagent corresponds to the complete activation of caspase 9 [ 14].
To determine the maximal fluorescence of the cells, ionomycin (final concentration 2 μ m) was added between the 40th and 41st frames.
For the plants, a FluorPen was used at the end of the experiments to measure the variable to maximal fluorescence of leaves (Fv Fm).
From the values of the fluorescence in the dark state (Fo), the fluorescence prior to hydroxylamine addition (F1), and the maximal fluorescence of the sample (F2), the percent retinal trapped was calculated by the expression [(F2 – F1)/(F2 – Fo)] × 100.
Fs is the steady state light adapted fluorescence, Fm' is the maximal fluorescence of a light adapted leaf following a saturating light pulse, and Fo' is the minimal fluorescence of a light adapted leaf that is darkened.
Similar(54)
The maximal quantum efficiency (Fv/Fm) of PSII was determined as the ratio of variable fluorescence (Fv) to maximum fluorescence of dark-adapted state (Fm).
Analysis of 4-D plots showed that vesicles often approach the PM and stop just below the point of maximal fluorescence for a fraction of a second (∼5 frames or 500 ms), and then fluorescence continues to increase.
Using a ratio of maximal fluorescence to basal fluorescence [ R F(max)/ F basal)] to compare basal and repressed transporter activity, individuals with low basal transporter activity exhibited a low R F(max)/ F basal), whereas animals with high basal activity had a high ratio.
The equation D = 0.88* w2/4t1/2) was used to calculate the diffusion coefficient for cytosolic Scyl1, where w = the radius of the circle ROI used during photobleaching, and t1/2 was the time of half maximal fluorescence recovery of GFP-Scyl1.
Camera gain was adjusted to give baseline maximal fluorescence levels of 40 100 (arbitrary units) of a maximal 8-bit signal output of 256.
Related(20)
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