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The maturation and replication of this virus entirely depend on the activity of a virus-encoded 3C protease.
HIV-1 protease (PR) is a major drug target in combating AIDS, as it plays a key role in maturation and replication of the virus.
The highly conserved nature of this protease across different viral strains and its crucial role in viral maturation and replication make it a very desirable target for inhibitor design.
To investigate the role of this disulfide bridge in virus maturation and replication, we engineered an M-PMV clone in which both protease cysteine residues were replaced by alanine M-PMVPRC7A/C106A M-PMVPRC7A/C106A
Pif1 is involved in several processes that are essential for DNA replication such as Okazaki fragment maturation and replication through G-quadruplex motifs and through replication fork barriers in rDNA (reviewed in ref (20)).
Malaria-associated morbidity and mortality is a result of the intra-erythrocytic developmental cycle (IDC) where repeated rounds of parasite invasion into red blood cells (RBCs) are followed by intracellular maturation and replication.
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In summary, the cellular cytoskeleton network is involved in the SARS-CoV maturation and possibly replication process.
The data reveal an essential contribution of a SPI1 effector to subsequent SCV maturation and bacterial replication and illuminate unanticipated intracellular communication between bacterial effectors deployed by distinct T3SSs.
Pseudotyped viruses produced in this system can mimic most stages of the HIV-1 life cycle, including viral protein production, assembly, release, maturation, entry, integration and replication without producing replication-competent viruses.
These iron-requiring proteins are abundantly present in mitochondria, cytosol, and nucleus; such proteins diversely function in electron transfer, ribosome maturation, DNA replication and repair, and cell cycle control (Kaplan et al., 2006; Ye and Rouault, 2010; White and Dillingham, 2012).
Quantification of NS6-7 and PTB intensity on a linear section of the image revealed that colocalization was more apparent in regions of the cytoplasm containing higher NS6-7 intensity (presumably more mature) replication complexes, revealing a possible link between cytoplasmic PTB and replication complex maturation (see highlighted replication complex in Fig 7a and b).
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development and replication
processing and replication
growth and replication
maturation and secretion
maturation and parturition
maturation and plant
maturation and ovulation
maturation and transport
maturation and closure
maturation and acidification
maturation and stability
maturation and fruit
maturation and mortality
maturation and organ
maturation and decline
maturation and survival
maturation and degeneration
maturation and retest
maturation and senescence
maturation and storage
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