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Individual 3-mm scaffolds were then punched out of the mats using a dermal biopsy punch.
Plates were incubated on ice for 75 min and membranes harvested on 0.3% polyethylenimine pretreated Wallac filter mats using a Tomtec Harvester MachII (Tomtec, Hamden, CT, USA).
The plates were harvested after 16 h onto glass-fiber filter mats using a Tomtec-Harvester-96 (Tomtec, Hamden, CT).
After 66 h, all cultures were pulsed with 1 µCi of [3H]thymidine and harvested 6 h later onto filter mats using a cell harvester (Packard, Meriden, CT).
The cells were harvested onto filter mats using a cell harvester, and the incorporation of radioactivity into DNA was measured by liquid scintillation in a Microbeta counter (Pharmacia, Uppsala, Sweden).
Cells were harvested onto filter mats using a PHD cell harvester (Brandel, Gaithersburg, MD, USA).
Similar(53)
XRD patterns were generated from nonwoven fibrous mat using a Rigaku D/Max ULTIMA 11 X-ray diffractometer (Japan).
Steamboat took the early advantage in the match-up, until Borne flipped him over and slammed him to the mat using a belly-to-belly suplex.
Sitting on the edge of the mat, using a folded fan as an instructor's baton, Matsumoto watches as volunteer Kimiko Shinomiya, 66, prepares to serve tea to Kajita and another woman.
Scanning Electron Microscopy of L. ochracea sheaths was done on freshly precipitated L. ochracea-rich Fe-mats using a Hitachi S-4700 FESEM at DBI Bioimaging (http://www.dbi.udel.edu/bioimaging/fesem.html) at the University of Delaware.
First, we characterized the microbial composition in an active freshwater Fe-oxidizing mat using a combination of SSU rDNA clone libraries, pyrotags, and DNA sequencing of individual cell filaments.
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