Individual 3-mm scaffolds were then punched out of the mats using a dermal biopsy punch.
Plates were incubated on ice for 75 min and membranes harvested on 0.3% polyethylenimine pretreated Wallac filter mats using a Tomtec Harvester MachII (Tomtec, Hamden, CT, USA).
The plates were harvested after 16 h onto glass-fiber filter mats using a Tomtec-Harvester-96 (Tomtec, Hamden, CT).
After 66 h, all cultures were pulsed with 1 µCi of [3H]thymidine and harvested 6 h later onto filter mats using a cell harvester (Packard, Meriden, CT).
The cells were harvested onto filter mats using a cell harvester, and the incorporation of radioactivity into DNA was measured by liquid scintillation in a Microbeta counter (Pharmacia, Uppsala, Sweden).
Cells were harvested onto filter mats using a PHD cell harvester (Brandel, Gaithersburg, MD, USA).
We tested whether ramet recruitment was different than mortality in transplanted mats using a two-sided t-test.
The mat uses a conductive powering technique, which is more efficient than inductive powering but requires direct contact between the devices and the charging pad.
The AirPower mat uses a new kind of charging standard that supports multi-device charging, which might explain why it's not shipping right away: It probably requires more work to bring it to full scale production, which is actually something that was reported as a rumor earlier today prior to this event.
After probe standardization, MAT uses a sliding window approach to calculate a MATscore for each window (e.g. 1KB genomic region) based on the standardized value of all the probes in the window.
