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Individual haploids expressing GAF582SupC with His (MATa) and Ura (MATα) markers were used for mating to generate MATa/α diploid which were selected on –His –Ura plates.

F1 virgin females were then cultured on LS food for 7 days prior to mating to generate the F2 population, which were also reared on the LS diet.

This plasmid was conjugated into the ribH2 strain by biparental mating to generate ribH1 + pribH1Amp.

This plasmid was conjugated into the ribH1 strain by biparental mating to generate ribH1+pribH1Km.

The resulting plasmid, pLS_sce, was confirmed by sequencing and conjugated into ribH2 strain by biparental mating to generate ribH2 + pLS_sce.

The resulting plasmid, pribH2, was conjugated into the ribH2 strain by biparental mating to generate ribH2 + pribH2.

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Similar(52)

For cell culture experiments, heterozygous PEX13Δ/+ C57BL/6J mice (background >F10 generation) were mated to generate PEX13+/+ (wild-type), PEX13Δ/+ and PEX13Δ/Δ (PEX13-null) pups within one litter.

Individuals were randomly mated to generate 100 animals within each of 6000 subsequent generations in order to generate LD between loci.

Where indicated, these G-CC null mice were crossed with Black Swiss outbred mice (NTac NIHBS, Taconic, Hudson, NY) and the resulting heterozygous mice mated to generate homozygous wild type (WT) controls and GC-C null littermates (F2 generation).

First V1−/− and A1−/− animals were mated to generate V1+/−A1+/− animals.

Wnt4+/−; Inhbb+/− double heterozygotes were mated to generate Wnt4−/−; Inhbb−/− double knockout mice.

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