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However we still need a way of accounting for this in the random selection of chromosomes which mate to generate offspring.
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For cell culture experiments, heterozygous PEX13Δ/+ C57BL/6J mice (background >F10 generation) were mated to generate PEX13+/+ (wild-type), PEX13Δ/+ and PEX13Δ/Δ (PEX13-null) pups within one litter.
Individuals were randomly mated to generate 100 animals within each of 6000 subsequent generations in order to generate LD between loci.
Where indicated, these G-CC null mice were crossed with Black Swiss outbred mice (NTac NIHBS, Taconic, Hudson, NY) and the resulting heterozygous mice mated to generate homozygous wild type (WT) controls and GC-C null littermates (F2 generation).
This plasmid was conjugated into the ribH1 strain by biparental mating to generate ribH1+pribH1Km.
First V1−/− and A1−/− animals were mated to generate V1+/−A1+/− animals.
This plasmid was conjugated into the ribH2 strain by biparental mating to generate ribH1 + pribH1Amp.
Wnt4+/−; Inhbb+/− double heterozygotes were mated to generate Wnt4−/−; Inhbb−/− double knockout mice.
The resulting plasmid, pribH2_W22A, was confirmed by sequencing and conjugated into ribH2 strain by biparental mating to generate ribH2 + pLS_sce.
Heterozygous CPD1 targeted mice were mated to generate CPD1 null mice (see Figure S1 for further details).
The resulting plasmid, pLS_sce, was confirmed by sequencing and conjugated into ribH2 strain by biparental mating to generate ribH2 + pLS_sce.
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