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By contrast, the second reported that although maternal imprinting of MEST was correctly erased in DNA from sperm of low concentration, methylation at an H19 sequence typically de novo methylated in spermatogenesis was incomplete in these samples [26].
These are consistent with the maternal imprinting of this region in the placenta (57).
A study comparing the lipid profile in XmO versus XpO Turner women suggests maternal imprinting of the X chromosome adversely affects the lipid profile compared to paternal imprinting [ 161].
The higher BPA dose disrupted the parental specific, monoallelic expression of the Snrpn, Igf2 and Kcnq1ot1 genes in a tissue-specific manner, and resulted in the biallelic expression of the paternally expressed Snrpn gene in the placenta, suggesting that maternal imprinting of Snrpn was disturbed before fertilization or that Snrpn was susceptible to loss of imprinting during early embryogenesis.
To test the effect of a dCTCF allele on the paternal imprinting of garnet, Dp(1 f LJ9, y+ g+/ X^Y males were crossed to y 1 z a g 53d / y 1 z a g 53d ; CTCF X / TM3, Sb Ser females, and the reciprocal cross with Dp(1 f LJ9, y+ g+/ X^X virgin females was performed to test the effect on the maternal imprinting of garnet.
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During the revision of this manuscript, the maternal imprint of FAM50B was also confirmed by other groups using similar validation methods [ 38, 39].
The normal maternal imprints of SNRPN and KCNQ1OT1 in the GV and MI oocytes were stably maintained in vitro up to the secondary oocyte stage (Geuns et al., 2003, 2007b; Table III).
That study also showed maternal imprinting to account for 2 5%and3 4%4% of the phenotypic variance of backfat and growth rate, respectively.
The risk of childhood asthma is also greater for infants with a maternal compared with a paternal history of asthma [ 180], indicating the possible importance of maternal imprinting (preferential expression of maternal genes in the fetus) [ 181, 182], as well as the influence of the maternal-fetal interface [ 171].
These dCTCF insulator sites, located between the centric heterochromatic imprinting center and the imprint marker gene garnet, could account for the sensitivity of the maternal imprint to dCTCF expression.
Both DNA methylation and histone modifications are required to establish the paternal and maternal imprinting during development of the germ cells and to maintain it after fertilisation [ 1- 4].
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