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For tumors with no matching blood sample, sex-matched pooled baselines from all blood samples were used to calculate log2-R ratios.
The matching blood sample for the tumour containing a mutation was not available for sequencing.
Germ-line DNA was prepared from either a matching blood sample or from normal tissue.
Germ-line DNA was prepared from either a matching blood sample or from normal tissue microdissected away from tumourtissue.
If the germline genotype is known (e.g. from a matching blood sample), the user should replace the corresponding BAF values by NA.
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We first examined cell lines established from tumors and matching blood samples from 27 melanoma patients.
DNA from matching blood samples was genotyped in duplicate using HumanHap550 Duo BeadChips.
We collected simultaneous milk and blood samples of 10 women at two times postpartum and additional milk samples without matching blood samples.
The sequencing results from 44 paired tumour and matching blood samples were used to search for differences that distinguished tumour mtDNA from blood mtDNA.
This allowed discrimination of germ line from somatic events for tumor samples of interest, including those tissues without matching blood samples.
All samples were from cancer cases and subdivided according to the following criteria: TAM (n=30): endometrial tumours from women who had taken tamoxifen; average age 69.4±11.2 years; 10 with matching blood samples.
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