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Exact(6)
Whenever possible we used a patient matched blood sample as a control.
Wherever matched blood sample is not available, the blood sample with similar coverage was used.
The variant was observed along with the normal allelic nucleotide in the electropherograms of tumor tissue and matched blood sample of the corresponding patient.
Observed loss of KIT allele in the corresponding case primarily represented neoplastic event since the matched blood sample was found to have 2 copies of KIT.
The AsCNAR (allele-specific copy-number analysis using anonymous reference) algorithm was applied to perform a "self-reference paired analysis", by comparing each culture and corresponding tumor tissue to the matched blood sample.
Fragment analysis was performed with Gene Mapper software (Life Technologies, USA) and data was analyzed using Coffalyser software (MRC, Holland) where DNA copy number ratios of tumor samples were computed using the matched blood sample.
Similar(54)
Exome sequencing and verification were performed for nine pairs of ESCC and the matched blood samples, followed by validation with additional samples using Sanger sequencing.
No matched blood samples from those patients were available.
All these mutations were not detected in matched blood samples.
Exome sequencing was performed for nine pairs of ESCC tumor samples and the matched blood samples.
Furthermore, IFNβ levels were significantly correlated with IFN gene signatures from matched blood samples.
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