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With the development of massive sequencing techniques and the availability of genome sequences, Genomic SELEX has the potential to be used as a very powerful selection procedure to discover genomic aptamers, i.e. sequences within RNAs that bind ligands with high affinity and are thus able to act as sensors or receptors within regulatory domains.
Genome sequences are being obtained at an increased rate with the systematic application of massive sequencing techniques.
miR-3151, which was first identified by massive sequencing techniques, is located within the first intron of the BAALC gene.
This level of genomic variability is unexpected considering the difficulty of distinguishing among strains using standard molecular techniques and underscores the resolution provided by massive sequencing techniques.
Continuous improvement of biotechnologies, progress of massive sequencing techniques and development of new technologies for high-throughput analysis and annotation of biomolecular sequences are generating a huge amount of biomolecular data and knowledge.
Massive sequencing techniques are among the new strategies used in functional genomics for gene discovery and molecular markers development in non-model organisms or in those species whose genomes have not been completely sequenced.
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Massive parallel sequencing techniques allow unprecedentedly rapid and economical DNA sequencing.
Our results clearly show that with the current low sequencing costs of massive parallel sequencing techniques, a SNP identification step is recommended even in situations where a large number of SNPs is already available.
For whole-genome analysis purposes, however, the ChIP technique is now combined with high-throughput techniques such as microarrays (ChIP-chip) or sequencing (ChIP-seq) by newly developed MPS (massive parallel sequencing) techniques.
However, the advent of extremely powerful massive parallel sequencing techniques will help to enlarge the picture from specific SM clusters to a genome-wide scale and will provide valuable insights how chromatin structure and histone modifications react to metabolic changes and to mutations or over-expression of crucial regulators.
The advantages of the massive parallel sequencing technique lie in its unbiased high-throughput detection of small RNAs at a genome-wide scale, even for low-abundance transcripts, and in its unparalleled ability in identifying novel RNA transcripts and modification of RNAs such as RNA editing.
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massive sequencing approaches
massive sequencing technologies
large scale sequencing techniques
massive hybridisation techniques
massive capture techniques
massive adaptation techniques
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