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However in the immediate future, with the development of massive sequencing systems for application at clinical diagnosis, it will be necessary to have clinical criteria to guide studies.
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46 The new systems of massive sequencing (http://www.454.com/; http://www.illumina.com/technology/sequencing_technology.ilmn) make it possible to obtain the complete genomic sequence or, alternatively, to obtain the complete sequence of all the exons of the genes of the genome in a single individual at a reasonable speed.
A massive sequencing effort will identify most of the human genetic glitches and dormant diseases that today are commonplace.
However, it requires massive sequencing effort with considerable costs.
Massive sequencing data can be overwhelming to examine.
We had just entered the era of massive sequencing.
The complete system is updated on a four-weekly basis following the update of UniprotKB [ 28], to include the predictions for the most recent releases of sequences, either curated entries from Swissprot or sequences automatically generated from massive sequencing programs in TrEMBL.
Actually, hundreds of thousands of DNA variants are detected in massive sequencing projects of genetic disorders.
The present result emphasizes the complementarities of both SuperSAGE and other massive sequencing approaches.
Standard libraries for massive sequencing were generated using the TruSeq RNA Sample Prep Kit (Illumina).
RNA-seq was performed at the Massive Sequencing Platform of Cantoblanco (CSIC-PCM, Madrid, Spain).
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