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In this study, the potentiality of agricultural by-products for mass multiplication of Beauveria bassiana was determined.
The information could help in the selection of fast developing lines and thus in the mass multiplication of this bioagent.
Plant tissue culture techniques offer a viable tool for the mass multiplication of identical plant material and the germplasm conservation of rare endangered plants.
In the present study, SSF was used for the determination of suitability of different agricultural by-products for mass multiplication of ENPF, Beauveria bassiana.
This study optimized various agricultural by-products as substrate medium in SSF study in expectation of their utilization in mass multiplication of ENPF, B. bassiana.
In this regard, the agro-industrial wastes, with rich nutrient source and a solid support to promote the growth of filamentous fungi, could be a good choice for mass multiplication of ENPF.
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As a result of the polyphagous ability and voracious nature of C. carnea in addition to its vast geographical distribution (New, 1975), there is easiness of mass multiplication (Araujo and Bichao, 1990) and tolerance to several pesticides (Hassan et al., 1985).
However, the market share of mycoinsecticides is very low compared to chemical insecticides, mainly due to lack of suitable mass multiplication techniques (Bhanu Prakash et al. 2008).
However, the success of ENPF as biocontrol agent depends on their amenability to easy and cheap mass multiplication.
The results clearly suggest for seed germination ecology of Z. armatum in order to formulate seed pre-treatments protocol for mass multiplication along with short germination period.
Moreover, this method is not economical since the collection of stem cuttings leads to the interruption of growth of the mother plant, resulting in a yield reduction which can be minimized by in vitro regeneration and mass multiplication.
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