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The present manuscript described a very reliable method for large scale multiplication of B. monnieri.
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This protocol provides a basis for future studies on mutation induction, genetic engineering, and could be applied for large-scale multiplication of this valuable plant.
Large-scale multiplication of exquisite and rare hybrids using tissue culture techniques has helped orchids occupy a position as one of the top ten cut flowers.
The protocol discussed could be effectively employed for large-scale multiplication of C. paniculatus.
> The improved micropropagation protocol discussed here could be exploited for large-scale multiplication of C. paniculatus, a threatened and medicinally important plant species.
This is key to successful large-scale multiplication of I. khasiana since this species does not propagate readily under natural conditions.
Its commercial application could be realized for the large-scale multiplication and supply to the State Forest Department.
A second method was developed from friable callus maintained over the long term with a view to large-scale multiplication.
In this paper, we rectify this shortcoming by reporting a rapid method for its large-scale multiplication.
Effects of four different media, i.e. (i) Murashige and Skoog (MS), (ii) Mitra et al., (iii) Knudson (KC) and (iv) Gamborg et al. (B5), were evaluated for large-scale multiplication by asymbiotic seed germination.
Through monitoring of large scale clearings.
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