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All together, we noted a higher MS-KIF18A mRNA levels in bone marrow of young animals that was decreased with age.

Mesenchymal stromal cells were isolated from bone marrow of young (21 50 years old; 4 samples) and elderly donors (53 85 years old; 4 samples) as described in our previous work [14], [16].

As expected, we observed co-localization of pγH2AX and 53BP1 at the site of DNA damage foci in the nucleus of all the four Chk1+/− erythroid populations, from least differentiated stage I to more differentiated stage IV erythroblasts isolated from the bone marrow of young non-anemic Chk1+/− mice (Figure 5, Table 1).

To investigate this hypothesis, bone marrow-resident monocytes were isolated directly from the bone marrow of young and old mice.

To investigate this, bone marrow-resident monocytes, which had developed in vivo, were isolated directly from bone marrow of young and old mice.

45 days later, the frequency of mature NK cells (CD27−CD11b+) among total NK cells was measured in the bone marrow of young or aged recipients.

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This presents a potential difficulty in assessing the bone marrow of younger patients and patients receiving bone marrow-stimulating factors after CR [26].

Prochymal is a preparation of mesenchymal stem cells, which are obtained from the bone marrow of healthy young adults.

Prochymal is a preparation of mesenchymal stem cells, which are obtained from the bone marrow of healthy young adult donors.

The MSC were isolated from bone marrow of a young pig and grown in basal media (BM) containing DMEM+10% fetal bovine serum and antibiotics.

To confirm and extend these results, we stained bone marrow and spleens of young and aged B6 mice with mAbs to molecules whose expression is known to be regulated during NK cell maturation.

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